薄叶卷柏总黄酮的纯化工艺研究-《武汉职业技术学院学报》

文章信息/Info

Title:: The Research of Purification Process of Total Flavonoids from Selaginella Delicatula

Author(s):: YUAN Qiao-yu GUO Qun WAN Jun-mei; Wuhan Polytechnic, Wuhan430074, China

Keywords:: selaginella delicatula; macroporous adsorption resin; total flavonoids; purification technology

摘要:: 建立了一种大孔树脂分离纯化薄叶卷柏中总黄酮的工艺。通过静态、动态吸附及解吸试验，以吸附率和解吸率为主要指标考察各因素对纯化工艺的影响。优选的纯化工艺为：以D-101型大孔树脂为吸附剂，上样液质量浓度3.910mg·mL-1，上样流速为2BV/h，最佳上样量为19.55mg·mL-1，水洗至洗脱液无色，再以6BV50%乙醇洗脱总黄酮，洗脱流速为3BV/h。所得提取物中总黄酮纯度达70.55%，收率为87.04%。该优选工艺稳定可行，适用于薄叶卷柏总黄酮的纯化分离。

Abstract:: The paper sets out to optimize the purification technology of total flavonoids from the Selaginella delicatula by Macro porous resin. The technological conditions of purification are examined by static and dynamic adsorption and desorption experiments, taking the adsorption rate and desorption rate as the main index. D-101 macroporous resin is found to have good adsorption and desorption effects. The optimal purification conditions are as follows：the column liquid concentration is 3.910mg·mL-1; the adsorption rate is at 2BV/h, with the loaded amount of 19.55 mg·mL-1; the sample is eluted by water and 50% ethanol of 6BV at a flow rate of 3BV/h， The concentration of total flavonoids rises to 70.55% after the purification, and the yield reaches 87.04%. This optimized process is stable, feasible and suitable for separation and purification of total flavonoids from Selaginella delicatula.